Clinicians in Canada are faced with a lack of tools needed to appropriately stratify patients that present with thyroid nodules. Thus, many patients undergoing screening for suspicion of thyroid cancer are exposed to invasive and often unnecessary diagnostic procedures. The project team has developed the ThyroSPEC™ assay, a highly accurate, cost-efficient mass spectrometry-based mutation detection panel that allows for the identification of the most prevalent mutations associated with thyroid cancer. This project aims to clinically validate and implement the current version of the ThyroSPEC™ assay in a routine diagnostic setting in Alberta.