Triticale, the only man-made crop, results from a recent hybrid between rye and wheat. Triticale is a high yield grain and biomass plant that has an excellent degree of adaptability to abiotic stress and resistance to numerous diseases plaguing other cereal crops in North America. The development of triticale as an industrial platform has been keenly welcomed by the triticale producers’ group as it will result in a renaissance of this crop. The goals of this project are to identify rye expressed sequence tags and development of an array-based tool to identify rye sequences expressed in triticale. The significance of this research is primarily due to triticale’s potential impact as a platform crop toward supplying biomass for renewable production of ethanol.